竹永 啓三

takenagasensei11

プロフィール
1977年3月 埼玉大学理工学部生化学科卒業
1979年3月 東京工業大学大学院総合理工学研究科修士課程生命化学専攻修了
1983年3月 名古屋大学大学院理学研究科博士課程分子生物学専攻修了

 

 研究歴
1980年11月 千葉県がんセンター研究局化学療法研究部勤務
1989年1月〜1991年1月 米国国立衛生研究所(NIH)がん研究所(NCI)留学
1999年11月〜12月 お茶の水女子大学大学院人間文化研究科 非常勤講師
2004年9月〜12月 スウェーデンウプサラ大学バイオメディカルセンター 神経科学研究部 招聘研究員
2005年12月〜2008年3月 千葉県がんセンター研究局 転移制御研究室 室長
2005年12月〜2008年3月 千葉大学大学院 医学薬学府 先端生命科学 分子腫瘍生物学講座 客員准教授
2008年4月〜 島根大学医学部生命科学講座腫瘍生物学 准教授

 

 研究業績
Original Papers
1 Hozumi M, Takenaga K, Tomida M, Okabe,J. Role of leukocytes in ascites in the production of factor(s) stimulating differentiation of mouse myeloid leukemia cells. Gann, 68: 643-652, 1977.
2 Tomida M, Takenaga K, Yamamoto Y, Hozumi M. Enhancement of double- stranded polynucleotides of production by cultured mouse peritoneal macrophages of differentiation-stimulating factor(s) for mouse myeloid leukemic cells. Biochem. J., 176: 665-669, 1978.
3 Hozumi M, Honma Y, Tomida M, Okabe J, Kasukabe T, Sugiyama K, Hayashi M, Takenaga K, Yamamoto Y. Induction of differentiation of myeloid leukemia cells with various chemicals. Acta Haematologica Japonica 42: 941-952, 1979. (Review)
4 Hozumi M, Umezawa T, Takenaga K, Ohno T, Shikita M, Yamane I. Characterization of factors stimulating differentiation of mouse myeloid leukemia cells from a Yoshida sarcoma cell line cultured in serum-free medium. Cancer Res., 39: 5127-5131, 1979.
5 Takenaga K, Hozumi M. Induction of chloroquine of differentiation of cultured mouse myeloid leukemia cells. Gann, 71: 141-145, 1980..
6 Takenaga K, Hozumi M, Sakagami Y. Effects of retinoids on induction of differentiation of cultured mouse myeloid leukemia cells. Cancer Res., 40: 914-919, 1980.
7 Honma Y, Takenaga K, Kasukabe T, Hozumi M. Induction of differentiation of cultured human promyelocytic leukemia cells by retinoids. Biochem. Biophys. Res. Commun., 95: 507-512, 1980.
8 Takenaga K, Honma Y. Hozumi M. Inhibition of differentiation of mouse myeloid leukemia cells by phenolic antioxidants and α-tocopherol. Gann, 72: 104-112, 1981.
9 Takenaga K, Honma Y, Kado J, Hozumi M. Production of differentiation- inhibiting factor in cultured mouse myeloid leukemia cells treated with retinoic acid. Cancer Res., 41: 1948-1953, 1981.
10 Takenaga K, Honma Y, Hozumi M. Cellular retinoid-binding proteins in cultured human and mouse myeloid leukemia cells. Cancer Lett., 13: 1-6, 1981.
11 Takenaga K. Stimulation by retinoic acid of prostaglandin production and its inhibition by tumor promoters in mouse myeloid leukemia cells. Gann, 72: 488-497, 1981.
12 Takenaga K, Honma Y, Kado J, Hozumi M. Mechanisms of inhibition of mouse myeloid leukemia cell differentiation by prostaglandin F2. Gann, 73: 175-183, 1982.
13 Takenaga K. Differences in inducibility of morphological differentiation of parental cells, selected variant cells and cells from spontaneous metastases of mouse neuroblastoma cells. Cancer Lett., 19: 41-46, 1983.
14 Takenaga K. Characterization of low- and high-metastatic clones isolated from a Lewis lung carcinoma. Gann, 75: 61-71: 1984.
15 Takenaga K. Enhanced metastatic potential of cloned low-metastatic Lewis lung carcinoma cells treated in vitro with dimethylsulfoxide. Cancer Res., 44: 1122-1127, 1984.
16 Takenaga K. Enhancement of lung-colonizing ability of cloned low-metastatic Lewis lung carcinoma cells by treatment with highly polar compounds. Int. J. Cancer, 34: 83-89, 1984.
17 Oguro M, Takagi T, Takenaga K. Dynamic analysis of changing features of tumor cells incubated with antitumor agents in vitro and its application for predictive activity assay of antitumor agents. Jpn. J. Cancer Res., 76: 131-141, 1985.
18 Takenaga K. Effect of butyric acid on lung-colonizing ability of cloned low-metastatic Lewis lung carcinoma cells. Cancer Res., 46: 1244-1249, 1986.
19 Takenaga K. Effects of 12-O-tetradecanoylphorbol-13-acetate on adhesiveness and lung-colonizing ability of Lewis lung carcinoma cells. Cancer Res., 46: 375-380, 1986.
20 Takenaga K. Effects of 8-bromoadenosine 3′:5′-cyclic monophosphate on proteolytic enzymes, adhesiveness and lung-colonizing ability of cloned low-metastatic Lewis lung carcinoma cells. Jpn. J. Cancer Res. (Gann), 77: 998-1004, 1986.
21 Takenaga K. Modification of the metastatic potential of tumor cells by drugs. Cancer Metastasis Rev., 5: 67-75, 1986. (Review)
22 Tokunaga K, Takeda K, Kamiyama K, Kageyama H, Takenaga K, Sakiyama S. Isolation of cDNA clones for mouse cytoskeletal γ-actin and differential expression of cytoskeletal actin mRNAs in mouse cells. Mol. Cell. Biol., 8: 3929-3933, 1988.
23 Takenaga K, Nakamura Y, Tokunaga K, Kageyama H, Sakiyama S. Isolation and characterization of a cDNA that encodes mouse fibroblast tropomyosin isoform 2. Mol. Cell Biol., 8: 5561-5565, 1988.
24 Takenaga K. Nakamura Y, Sakiyama S. Suppression of synthesis of tropomyosin isoform 2 in metastatic v-Ha-ras-transformed NIH3T3 cells. Biochem. Biophys. Res. Commun., 157: 1111-1116, 1988.
25 Takenaga K, Nakamura Y, Sakiyama S. Differential expression of a tropomyosin isoform in low- and high-metastatic Lewis lung carcinoma cells. Mol. Cell. Biol., 8: 3934-3937, 1988.
26 Takenaga K, Nakamura Y, Kageyama H, Sakiyama S. Nucleotide sequence of cDNA for nonmuscle tropomyosin 5 of mouse fibroblast. Biochim. Biophys. Acta, 1087: 101-103, 1990.
27 Nakanishi H, Oguri K, Yoshida K, Itano N, Takenaga K, Kazama T, Yoshida A, Okayama M. Structural differences between heparan sulfate of proteoglycan involved in the formation of basement membranes in vivoby Lewis-lung- carcinoma-derived cloned cells with different metastatic potentials. Biochem. J., 288: 215-224, 1992.
28 Nakanishi H, Takenaga K, Oguri K, Yoshida A, Okayama M. Morphological characteristics of tumors formed by Lewis lung carcinoma-derived cloned cell lines with different metastatic potentials: structural differences in their basement membranes formed in vivo. Virchows Archiv A Pathol. Anat., 420: 163-170, 1992.
29 Takenaga K, Nakamura Y, Sakiyama S, Hasegawa Y, Sato K, Endo H. Binding of pEL98, an S100-related calcium-binding protein, to nonmuscle tropomyosin. J. Cell Biol., 124: 757-768, 1994. .
30 Takenaga K, Nakamura Y, Endo H, Sakiyama S. Involvement of pEL98, an S100-related calcium-binding protein, in cell motility and tumor cell invasion. Jpn. J. Cancer Res., 85: 831-839, 1994.
31 Takenaga K, Nakamura Y, Sakiyama S. Cellular localization of pEL98 protein, an S100-related calcium binding protein, in fibroblasts and its tissue distribution analyzed by monoclonal antibodies. Cell Struct. Funct., 19: 133-141, 1994.
32 Takenaga K, Masuda A. Restoration of microfilament bundle organization in v-raf-transformed NRK cells transduced with tropomyosin 2 cDNA. Cancer Lett., 87: 47-53, 1994.
33 Takenaga, K., Nakamura, Y. and Sakiyama, S. Cellular localization of pEL98 protein, an S100-related calcium binding protein, in fibroblasts and its tissue distribution analyzed by monoclonal antibodies. Cell Struct. Funct., 19, 133-141, 1994.
34 Nakanishi H, Oguri K, Takenaga K, Hosoda S, Okayama M. Differential fibrotic stromal responses of host tissue to low- and high-metastatic cloned Lewis lung carcinoma cells. Lab. Invest., 70: 324-332, 1994.
35 Nakamura Y, Sakiyama S, Takenaga K. Suppression of syntheses of high molecular weight nonmuscle tropomyosins in macrophages. Cell Motil. Cytoskeleton, 31: 273-282, 1995.
36 Ozaki T, Ma J, Takenaga K, Sakiyama S. Cloning of mouse DAN cDNA and its down-regulation in transformed cells. Jpn. J. Cancer Res., 87: 58-61, 1996.
37 Gunji Y, Tagawa M, Matsubara H, Takenaga K, Shimada H, Kondo F, Suzuki T, Nakajima K, Sugaya M, Asano T, Ochiai T, Isono K, Horitsu K, Kageyama H, Nakamura Y, Sakiyama S. Antitumor effect induced by the expression of granulocyte macrophage-colony stimulating factor gene in murine colon carcinoma cells. Cancer Lett., 101: 257-261, 1996.
38 Gunji Y, Tagawa M, Matsubara H, Takenaga K, Shimada H, Kondo F, Suzuki T, Nakajima K, Aoki T, Asano T, Ochiai T, Isono K, Kageyama H, Nakamura Y, Sakiyama S. Murine colon carcinoma cells engineered to produce human interleukin-2 induce tumor specific antitumor response. Int. J. Cancer, 66: 135-139, 1996.
39 Matsubara H, Tagawa M, Gunji Y, Takenaga K, Sugaya M, Urashima T, Koide Y, Suzuki T, Asano T, Ochiai T, Isono K, Kageyama H, Nakamura Y, Sakiyama S. Study of irradiation effect on cytokine secretion from retrovirally-transduced tumor cells: A model for tumor vaccination. Anticancer Res., 16: 645-650, 1996.
40 Masuda A, Takenaga K, Kondoh F, Fukami H, Utsumi K, Okayama H. Role of a signal transduction pathway which controls disassembly of microfilament bundles and suppression of high-molecular-weight tropomyosin expression in oncogenic transformation of NRK cells. Oncogene, 12: 2081-2088, 1996.
41 Iwadate Y, Namba H, Tagawa M, Takenaga K, Sueyoshi K, Sakiyama S. In vivo bystander effect in the intracranical model with rat glioma cells reflects the clonal difference of HSV-TK positive cells. Int. J. Oncol., 9: 521-525, 1996.
42 Takenaga K, Nakamura Y, Tagawa M, Kageyama H, Sakiyama, S. Augmentation of in vivo growth of Lewis lung carcinoma cells transduced with granulocyte- macrophage colony-stimulating factor gene. Cancer Lett., 105: 33-37, 1996.
43 Takenaga K, Tagawa M, Sakiyama S. Therapeutic potency of transduction with herpes simplex virus thymidine kinase gene against multidrug resistant mouse leukemia cells. Anticancer Res., 16: 681-686, 1996.
44 Takenaga K. Suppression of metastatic potential of high-metastatic Lewis lung carcinoma cells by vanadate, an inhibitor of tyrosine phosphatase, through inhibiting cell-substrate adhesion. Invasion Metastasis, 16: 97-106, 1996.
45 Gunji Y, Tagawa M, Matsubara H, Takenaga K, Sugaya M, Tasaki K, Maeda T, Kondo F, Nakajima K, Suzuki T, Asano T, Ochiai T, Isono K, Sakiyama S. Inhibition of peritoneal dissemination of colon carcinoma in syngeneic mice immunized with interleukin-2-producing cells. Cancer Lett., 109: 171-176, 1996.
46 Iwadate Y, Namba H, Tagawa M, Takenaga K, Sueyoshi K, Sakiyama S. Induction of acquired immunity in rats that have eliminated intracranical gliosarcoma cells by the expression of herpes simplex virus-thymidine kinase gene and ganciclovir administration. Oncology, 54: 329-334, 1997.
47 Kimura M, Tagawa M, Takenaga K, Yamaguchi T, Saisho H, Nakagawara A, Sakiyama S. Inability to induce the alteration of tumorigenicity and chemosensitivity of p53-null human pancreatic carcinoma cells after the transduction of wild-type p53 gene. Anticancer Res., 17: 879-883, 1997.
48 Gunji Y, Tagawa M, Matsubara H, Takenaga K, Shimada H, Kondo F, Suzuki T, Nakajima K, Sugaya M, Asano T, Ochiai T, Isono K, Kageyama H, Nakamura Y, Sakiyama S. Antitumor effect of murine colon carcinoma cells retrovirally transduced with interleukin-4 and granulocyte macrophage-colony stimulating factor genes. Oncology, 54: 69-73, 1997.
49 Kimura M, Tagawa M, Takenaga K, Nakagawara A, Horitsu K, Yamaguchi T, Saisho H, Sakiyama S. Drug resistance to ganciclovir observed in suicide gene therapy is due to the loss of integrated herpes simplex virus-thymidine kinase gene. Int. J. Oncology, 10: 775-778, 1997.
50 Tada A, Kato H, Takenaga K, Hasegawa S. Transforming growth factor β1 increases the expressions of high-molecular weight tropomyosin isoforms and vinculin and suppresses the transformed phenotypes in human lung carcinoma cells. Cancer Lett, 121: 31-37, 1997.
51 Takenaga K, Nakamura Y, Sakiyama S. Expression of antisense RNA to S100A4 gene encoding an S100-related calcium-binding protein suppresses metastatic potential of high-metastatic Lewis lung carcinoma cells. Oncogene, 14: 331-337, 1997.
52 Takenaga K, Nakanishi H, Wada K, Suzuki M, Matsuzaki O, Matsuura A, Endo H. Increased expression of S100A4, a metastasis-associated gene, in human colorectal adenocarcinomas. Clin. Cancer Res., 3: 2309-2316, 1997.
53 Tasaki K, Yoshida Y, Tagawa M, Takenaga K, Asano T, Ochiai T, Isono K, Kouzu T, Saisho H, Sakiyama S. Discordant production of released exogenous protein and infectious virions from retrovirus-packaging cells used for gene transduction. Anticancer Res., 17: 4415-4418, 1997.
54 Sugaya M, Tagawa M, Matsubara H, Gunji Y, Takenaga K, Maeda T, Koide Y, Asano T, Ochiai T, Isono K, Sakiyama S. Induction of antitumor effect on human esophageal carcinoma cells by the retroviral expression of granulocyte macrophage-colony stimulating factor gene. Int. J. Oncol., 12: 321-324, 1998.
55 Tagawa M, Goto S, Takenaga K, Takeshita A, Saotome T, Takenouchi T, Tsurumachi T, Gunji Y, Matsubara H, Sakiyama S. Reduced tumorigenicity of human gastric carcinoma cells engineered to produce IL-2 in SCID mice reconstituted with peripheral blood cells from cancer patients. Cancer Lett., 123: 87-93, 1998.
56 Yoshida H, Enomoto H, Miyauchi M, Takenaga K, Tanabe M, Ohnuma N, Sakiyama S, Tagawa M. Impaired tumorigenicity of IL-4-producing murine neuroblastoma cells in immunodeficient mice. Int. J. Oncology, 12: 1067-1071, 1998.
57 Yoshida Y, Tasaki K, Kimura M, Takenaga K, Yamamoto H, Yamaguchi T, Saisho H, Sakiyama S, Tagawa M. Antitumor effect of human pancreatic cancer cells transduced with cytokine genes which activate Th1 helper T cells. Anticancer Res., 18: 333-336, 1998.
58 Kimura M, Tagawa M, Yoshida Y, Takenouchi T, Takenaga K, Azuma K, Yamaguchi T, Saisho H, Sakiyama S. Impaired in vivo growth of human pancreatic carcinoma cells retrovirally transduced with GM-CSF gene. Anticancer Res., 18: 165-170, 1998.
59 Yoshida H, Enomoto H, Tagawa M, Takenaga K, Tasaki K, Nakagawara A, Ohnuma N, Takahashi H, Sakiyama S. Impaired tumorigenicity and decreased liver metastasis of murine neuroblastoma cells engineered to secrete interleukin-2 or granulocyte macrophage colony-stimulating factor. Cancer Gene Ther., 5: 67-73, 1998.
60 Tasaki K, Tagawa M, Gunji Y, Matsubara H, Takenaga K, Muramatsu M, Fujimura S, Suzuki T, Asano T, Ochiai T, Isono K, Kouzu T, Sakiyama S. Inhibition of experimental lung metastasis of murine colon carcinoma cells depends on the amount of interleukin-2 secreted from the transformed cells. Anticancer Res., 18: 813-818, 1998.
61 Duarte WR, Kasugai S, Iimura T, Oida S, Takenaga K, Ohya K, Ishikawa I. cDNA cloning of S100 calcium-binding proteins from bovine periodontal ligament and their expression in oral tissues. J. Dental Res., 77: 1694-1699, 1998.
62 Tasaki K, Gunji Y, Matsubara H, Takenaga K, Suzuki T, Asano T, Ochiai T, Isono K, Kouzu T, Sakiyama S, Tagawa, M. Induction of T cell dependent acquired immunity in syngeneic mice by the combined expression of interleukin-4 and granulocyte macrophage-colony stimulating factor gene in murine colon carcinoma cells. Anticancer Res., 18: 1453-1456, 1998.
63 Kimura M, Tagawa M, Takenaga K, Kondo F, Yamaguchi T, Saisho H, Nakagawara A, Sakiyama S. Loss of tumorigenicity of human pancreatic carcinoma cells engineered to produce interleukin-2 or interleukin-4 in nude mice: A potentiality for cancer gene therapy. Cancer Lett, 128: 47-53, 1998.
64 Nakamura N, Takenaga K. Hypomethylation of the metastasis-associated S100A4 gene correlates with gene activation in human colon adenocarcinoma cell lines. Clin. Exp. Metastasis, 16: 471-479, 1998.
65 Yoshida H, Enomoto H, Kawamura K, Takenaga K, Tanabe M, Ohnuma N, Sakiyama S, Tagawa M. Antitumor vaccine effect of irradiated murine neuroblastoma cells producing interleukin-2 or granulocyte macrophage-colony stimulating factor. Int. J. Oncol, 13: 73-78, 1998.
66 Lee J-Y, Kambe M, Hayashi M, Takenaga K. Cloning and characterization of a novel zinc finger protein that associated with nuclear matrix. DNA Cell Biol., 17: 849-858, 1998.
67 Gunji Y, Tasaki K, Tagawa M, Matsubara H, Takenaga K, Suzuki T, Asano T, Ochiai T, Isono K, Sakiyama S. Inhibition of peritoneal dissemination of murine colon carcinoma cells by administrating retrovirus harboring IL-2 gene. Cancer Gene Ther., 5: 339-343, 1998.
68 Takenaga K. Suppression of metastasis-associated S100A4 gene expression by γ-interferon in human colon adenocarcinoma cells. Brit. J. Cancer, 80: 127-132, 1999.
69 Duarte WR, Mikuni-Takagaki Y, Kawase T, Limura T, Oida S, Ohya K, Takenaga K, Ishikawa L, Kasugai S. Effects of mechanical stress on the mRNA expression of S100A4 and cytoskeletal components by periodontal ligament cells. J. Med. Dent. Sci., 46: 117-122, 1999.
70 Duarte WR, Iimura T, Oida S, Takenaga K, Ohya K, Ishikawa I, Kasugai S. Extracellular role of S100A4 calcium-binding protein in the periodontal ligament. Biochem. Biophys. Res. Commun. 255: 416-420, 1999.
71 Matsubara H, Kimura M, Sugaya M, Koide Y, Gunji Y, Takenaga K, Asano T, Ochiai T, Isono K, Sakiyama S, Tagawa M. Expression of wild-type p53 gene confers increased sensitivity to radiation and chemotherapeutic agents in human esophageal carcinoma cells. Int. J. Oncol, 14: 1081-1085, 1999.
72 Miyauchi M, Shimada H, Kadomatsu K, Muramatsu T, Matsubara S, Ikematsu S, Takenaga K, Asano T, Ochiai T, Sakiyama S, Tagawa M. Frequent expression of midkine gene in esophageal cancer suggests a potential usage of its promoter for suicide gene therapy. Jpn. J. Cancer Res., 90: 469-475, 1999.
73 Kimura M, Yoshida Y, Narita M, Takenaga K, Takenouchi T, Saisho H, Sakiyama S, Tagawa M. Acquired immunity in nude mice induced by expression of the IL-2 or IL-4 gene in human pancreatic carcinoma cells and anti-tumor effect generated by in vivo gene transfer using retrovirus. Int. J. Cancer, 82: 549-555, 1999..
74 Yoshida H, Tanabe M, Miyauchi M, Kawamura K, Takenaga K, Ohnuma N, Sakiyama S, Tagawa M. Induced immunity by expression of interleukin-2 or GM-CSF gene in murine neuroblastoma cells can generate antitumor response to established tumors. Cancer Gene Ther., 6: 395-401 1999.
75 Narita M, Takenaga K, Yoshida Y, Kadomatsu K, Muramatsu T, Matsubara S, Hamada H, Goto S, Saisho H, Sakiyama S, Tagawa M. Polyadenylation signal facilitates the expression of foreign gene that is driven by an internal promoter located in the reverse orientation to long terminal repeat of retrovirus. Anticancer Res., 20: 279-282, 2000.
76 Takasu M, Tada Y, Wang JO, Tagawa M, Takenaga K. Resistance to apoptosis induced by microenvironmental stresses is correlated with metastatic potential in Lewis lung carcinoma. Clin. Expt. Metastasis, 17: 409-416, 1999.
77 Matsubara H, Kawamura K, Sugaya M, Koide Y, Gunji Y, Takenaga K, Asano T, Ochiai T, Sakiyama S, Tagawa M. Differential efficacy of suicide gene therapy by herpes simplex virus-thymidine kinase gene reflects the status of p53 gene in human esophageal cancer cells. Anticancer Res., 19: 4157-4160, 1999.
78 Tasaki K, Yoshida Y, Maeda T, Miyauchi M, Kawamura K, Takenaga K, Yamamoto H, Kouzu T, Asano T, Ochiai T, Sakiyama S, Tagawa M. Protective immunity is induced in murine colon carcinoma cells by the expression of interleukin-12 or interleukin-18 which activate Th1-type helper T cells. Cancer Gene Ther., 7: 247-254, 2000.
79 Tasaki K, Yoshida Y, Miyauchi M, Maeda T, Takenaga K, Kouzu T, Asano T, Ochiai T, Sakiyama S, Tagawa M. Transduction of murine colon carcinoma cells with interleukin-15 gene induces antitumor effect in immunocompromised hosts. Cancer Gene Ther., 7: 255-261, 2000.
80 Yoshida Y, Tasaki K, Miyauchi M, Narita M, Takenaga K, Yamamoto H, Yamaguchi T, Saisho H, Sakiyama S, Tagawa M. Impaired tumorigenicity of human pancreatic cancer cells retrovirally transduced with interleukin-12 or interleukin-15 gene. Cancer Gene Ther., 7: 324-331, 2000.
81 Koshikawa N, Takenaga K, Tagawa M, Sakiyama S. Therapeutic efficacy of the suicide gene driven by the promoter of vascular endothelial growth factor gene against hypoxic tumor cells. Cancer Res., 60: 2936-2941, 2000.
82 Lee J-Y, Nakane Y, Koshikawa N, Nakayama K, Hayashi M, Takenaga K. Characterization of a zinc finger protein ZAN75: Nuclear localization signal, transcriptional activator activity, and expression during neuronal differentiation of P19 cells. DNA Cell Biol., 19: 227-234, 2000.
83 Kawamura K, Tasaki K, Hamada H, Takenaga K, Sakiyama S, Tagawa M. Expression of Eschericia coli uracil phosphoribosyltransferase gene in murine colon carcinoma cells augments the antitumor effect of 5-fluorouracil and induces protective immunity. Cancer Gene Ther., 7: 637-643, 2000.
84 Kawamura K, Bahar R, Namba H, Seimiya M, Takenaga K, Hamada H, Sakiyama S, Tagawa M. Bystander effect in uracil phosphoribosyltransferase/5-fluorourasil- mediated suicide gene therapy is correlated with the level of intracellular communication. Int. J. Oncol, 18: 117-120, 2001.
85 Tada Y, O-Wang J, Takenaga K, Takiguchi Y, Tatsumi K, Kuriyama T, Tagawa M. Expression of the TNF-α gene on mouse lung carcinoma cells suppresses spontaneous lung metastasis without affecting tumorigenicity. Oncol. Rep., 9: 585-588, 2002.
86 Endo H*, Takenaga K*, Kanno T, Satoh H, Mori S. Methionine aminopeptidase 2 is a new target for the metastasis-associated protein, S100A4. J. Biol. Chem., 277: 26396-26402, 2002. (*equal contribution)
87 Enomoto N, Koshikawa N, Gassmann M, Hayashi J-I, Takenaga K. Hypoxic induction of hypoxia-inducible factor-1α and oxygen-regulated gene expression in mitochondrial DNA-depleted HeLa cells. Biochem. Biophys. Res. Commun., 297: 346-352, 2002.
88 Koshikawa N, Iyozumi A, Gassmann M, Takenaga K. Constitutive upregulation of hypoxia-inducible factor-1α mRNA occurring in highly metastatic lung carcinoma cells leads to vascular endothelial growth factor overexpression upon hypoxic exposure. Oncogene, 22: 6717-6724, 2003.
89 Oga M, Takenaga K, Sato Y, Nakajima H, Koshikawa N, Osato K, Sakiyama S. Inhibition of metastatic brain tumor growth by intramuscular administration of the endostatin gene. Int. J. Oncol, 23: 73-79, 2003.
90 Tada Y, O-Wang J, Yu L, Shimozato O, Wang YQ, Takiguchi Y, Tatsumi K, Kuriyama T, Takenaga K, Sakiyama S, and Tagawa M. T-cell-dependent antitumor effects produced by CD40 ligand expressed on mouse lung carcinoma cells are linked with the maturation of dendritic cells and secretion of a variety of cytokines. Cancer Gene Ther., 10: 451-456, 2003.
91 Duarte WR, Shibata T, Takenaga K, Takahashi E, Kubota K, Ohya K, Ishikawa Y, Yamauchi M, Kasugai S. S100A4: a novel negative regulator of mineralization and osteoblast differentiation. J. Bone Miner. Res., 18: 493-501, 2003.
92 Wang Y, Seimiya M, Kawamura K, Yu L, Ogi T, Takenaga K, Shishikura T, Nakagawara A, Sakiyama S, Tagawa M, O-Wang J. Elevated expression of DNA polymerase κ in human lung cancer is associated with p53 inactivation: Negative regulation of POLK promoter activity by p53. Int J Oncol., 25: 161-165, 2004.
93 Kato C, Kojima T, Komaki M, Mimori K, Duarte WR, Takenaga K, Ishikawa I. S100A4 inhibition by RNAi up-regulates osteoblast related genes in periodontal ligament cells. Biochem Biophys Res Commun., 326: 147-153, 2005..
94 Kondo N, Ichimiya S, Tamura Y, Tonooka A, Koshiba S, Torigoe T, Kamiguchi K, Takenaga K, Sato N. A calcium binding protein, S100A4, mediates T cell dependent cytotoxicity as a transformation-associated antigen. Microbiol Immunol., 49: 49-56, 2005.
95 Kozlova EN, Takenaga K. A procedure for culturing astrocytes from white matter, and the application of the siRNA technique for silencing the expression of their specific marker, S100A4. Brain Res Brain Res Protoc., 15: 59-65, 2005.
96 Koshikawa N, Takenaga K. Hypoxia-regulated expression of attenuated diphtheria toxin a fused with HIF-1α oxygen-dependent degradation domain preferentially induced apoptosis of hypoxic cells in solid tumor. Cancer Res., 65: 11622-11630, 2005.
97 Takenaga K, Kozlova EN. The role of intracellular S100A4 for migration of rat astrocytes. Glia, 53: 313-321, 2006.
98 Koshikawa N, Maejima C, Miyazaki K, Nakagawara A, Takenaga K. Hypoxia selects for high-metastatic Lewis lung carcinoma cells overexpressing Mcl-1 and exhibiting reduced apoptotic potential in solid tumors. Oncogene, 25: 917-928, 2006.
99 Fang Z, Forslund N, Takenaga K, Lukanidin E, Kozlova EN. Sensory neurite outgrowth on white matter astrocytes is influenced by intracellular and extracellular S100A4 protein. J. Neurosci. Res., 83: 619-626, 2006.
100 Fang Z, Duthoit N, Wicher G, Kallskog O, Ambartsumian N, Lukanidin E, Takenaga K, Kozlova EN. Intracellular calcium-binding protein S100A4 influences injury-induced migration of white matter astrocytes. Acta Neuropathol. (Berl), 111: 213-219, 2006.
101 Ito A, Koshikawa N, Mochizuki S., Omura K, Takenaga K. Hypoxia-inducible factor-1 mediates the expression of DNA polymerase ι. Biochem. Biophys. Res. Commun., 351: 306-311, 2006.
102 Takenaga K, Nygren J, Zelenina M, Ohira M, Iuchi T, Lukanidin E, Sjoquist, Kozlova EN. Modified expression of S100A4/Mts1 protein in C6 glioma cells or surrounding astrocytes affects migration of tumor cells in vitro and in vivo. Neurobiol. Dis., 25: 455-463, 2007.
103 Ito S, Koshikawa N, Mochizuki S, Takenaga K. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. Int. J. Oncol., 31: 261-268, 2007.
104 Ishikawa K*, Takenaga K*, Akimoto M, Koshikawa N, Yamaguchi A, Imanishi H, Nakada K, Honma Y, Hayashi J. ROS-generating mitochondrial DNA mutations can regulate tumor cell metastasis. Science, 320: 661-664, 2008. (*equal contribution)
105 Ishikawa K, Koshikawa N, Takenaga K, Nakada K, Hayashi J. Reversible regulation of metastasis by ROS-generating mtDNA mutations. Mitochondrion, 8: 339-344, 2008.
106 Ishikawa K, Hashizume O, Koshikawa N, Fukuda S, Nakada K, Takenaga K, Hayashi J. Enhanced glycolysis induced by mtDNA mutations does not regulate metastasis. FEBS Lett., 582: 3525-3530, 2008.
107 Koshikawa N, Hayashi J, Nakagawara A, Takenaga K. Reactive oxygen species-generating mitochondrial DNA mutation up-regulates hypoxia-inducible factor-1α gene transcription via phosphatidylinositol 3-kinase-Akt/protein kinase C/histone deacetylase pathway. J. Biol. Chem., 284: 33185-33194, 2009.
108 Kojima S, Hyakutake A, Koshikawa N, Nakagawara A, Takenaga K. MCL-1V, a novel mouse antiapoptotic MCL-1 variant, generated by RNA splicing at a non-canonical splicing pair. Biochem. Biophys. Res. Commun., 391: 492-497, 2010.
109 Ishikawa K, Toyama-Sorimachi N, Nakada K, Morimoto M, Imanishi H, Yoshizaki M, Sasawatari S, Niikura M, Takenaga K, Yonekawa H, Hayashi J. The innate immune system in host mice targets cells with allogenic mitochondrial DNA. J Expt. Med. 207: 2297-2305, 2010.
110 Yokota M, Shitara H, Hashizume O, Ishikawa K, Nakada K, Ishii R, Taya C, Takenaga K, Yonekawa H, Hayashi J. Generation of trans-mitochondrial mito-mice by the introduction of a pathogenic G13997A mtDNA from highly metastatic lung carcinoma cells. FEBS Lett. 584: 3943-3948, 2010.
111 Takahashi T, Kawakami K, Mishima S, Akimoto M, Takenaga K, Suzumiya J, Honma Y. Cyclopamine induces eosinophilic differentiation and upregulates CD44 expression in myeloid leukemia cells. Leukemia Res., 35: 638-645, 2011.
112 Imanishi H, Hattori K, Wada R, Ishikawa K, Fukuda S, Takenaga K, Nakada K, Hayashi J. Mitochondrial DNA mutations regulate metastasis of human breast cancer cells. PLoS One. 6: e23401, 2011.
113 Takenaga K. Angiogenic signaling aberrantly induced by tumor hypoxia. Front. Biosci., 16: 31-48, 2011. (Review)
114 Kai S, Tanaka T, Daijo H, Harada H, Kishimoto S, Suzuki K, Takabuchi S, Takenaga K, Fukuda K, Hirota K. Hydrogen sulfide inhibits hypoxia- but not anoxia-induced hypoxia-inducible factor 1 activation in a von Hippel-Lindau- and mitochondria-dependent manner. Antioxid Redox Signal. 16: 203-216, 2012.
115 Kawakami K, Hattori M, Inoue T, Maruyama Y, Ohkanda J, Kato N, Tongu M, Yamada T, Akimoto M,Takenaga K, Sassa T, Suzumiya J, Honma Y. A novel fusicoccin derivative preferentially targets hypoxic tumor cells and inhibits tumor growth in xenografts. Anticancer Agents Med Chem. 12: 791-800, 2012.
116 Hashizume O, Shimizu A, Yokota M, Sugiyama A, Nakada K, Miyoshi H, Itami M, Ohira M, Nagase H,Takenaga K, Hayashi J. Specific mitochondrial DNA mutation in mice regulates diabetes and lymphoma development. Proc Natl Acad Sci U S A. 109: 10528-10533, 2012.
117 Nishi T, Maruyama R, Urano T, Nakayama N, Kawabata Y, Yano S, Yoshida M, Nakayama K, Miyazaki K,Takenaga K, Tanaka T, Tajima Y. Low expression of nucleus accumbens-associated protein 1 predicts poor prognosis for patients with pancreatic ductal adenocarcinoma. Pathol Int. 62: 802-810, 2012.
118 Ishikawa K, Imanishi H, Takenaga K, Hayashi J. Regulation of metastasis; mitochondrial DNA mutations have appeared on stage. J Bioenerg Biomembr. 44: 639-644, 2012. (Review)
119 Shimojo Y, Akimoto M, Hisanaga T, Tanaka T, Tajima Y, Honma Y, Takenaga K. Attenuation of reactive oxygen species by antioxidants suppresses hypoxia-induced epithelial-mesenchymal transition and metastasis of pancreatic cancer cells. Clin Exp Metastasis. 30: 143-154, 2013.
120 Hattori M, Kawakami K, Akimoto M, Takenaga K, Suzumiya J, Honma Y. Antitumor effect of Japanese apricot extract (MK615) on human cancer cells in vitro and in vivo through a reactive oxygen species-dependent mechanism. Tumori, 99: 239-248, 2013.